I hypothesize that in the cultures used in this lab for gram staining will contain many gram negative and gram positive bacteria. One distinct colony grew and a Gram stain was performed on the isolated colony. This it stains cell walls purple Iodine when combined with a mordant.
It is a brown basic diazo dye that is 1,2, 1, 2… up to the last student used as a biological stain. Flood the crystal violet for one minute. Bacteria was categorised into two groups in by the Danish Bacteriologist Christian, gram positive and gram negative by a staining technique where the ability to avoid de-coloration of Crystal Violet solution by alcohol would render the category of gram positive, and gram negative if the bacteria is de-coloured.
Place the slide under microscope on low powered lens. Iodine is often referred to as a mordant, but is a trapping agent that prevents the removal of the CV—I complex and, therefore, color the cell. There are four basic steps of the Gram stain: Used to detect proteins after electrophoretic separation on polyacrylamide gels.
Gram Stain to color cells, tissues, components or metabolic process.
Under oil immersion the cells became more sparse and under the high powered lens of the microscope spores could be seen, as little bubbles, in the cells.
Will you read function number 5. Ammonium Oxalate-Crystal Violet stain 5. This rule is followed by two phyla—Firmicutes except for the classesMollicutes and Negativicutes and the Actinobacteria.
It includes many well-known genera such as Bacillus, Listeria,Staphylococcus, Streptococcus, Enterococcus, and Clostridium.
The speed of de-coloration was not inspected very clearly thus no further conclusion could be reached, yet it is possible that this an old culture of Bacilli shaped Gram Positive bacteria.
October Gram-indeterminate bacteria do not respond to Gram staining and, therefore, cannot be determined as either Gram-positive or Gram-negative. Read about staining techniques in Tortora et. What is the use of special stains. Draw the cells showing morphology, grouping, and relative sizes.
We came to answer in the table from which we will paste the common stains beside the description or their uses. Move to higher powered lens, repeat step 3 6. Match the descriptions in column A with the stain in column B. Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining.
Gram Stains is used to identify and classify bacteria, it is a differential stain that allows you to classify bacteria as either gram-positive or gram-negative. The foloowing is a breif summerary of the gram staining procedure. Gram staining is a differential or specific method of staining.
Gram staining is a way to tell the difference between gram positive and gram negative bacteria. The cell wall of the gram positive bacteria is made up of several “layers of peptidoglygan,” and “techoic acids,” (LabPaq, p. ). If you need a custom term paper on Biology: Bacteria Classification By Gram Staining, you can hire a professional writer here to write you a high quality authentic essay.
While free essays can be traced by Turnitin (plagiarism detection program), our custom written essays will pass any plagiarism test. Results The gram staining lab was a huge success.
Many different types of both gram negative and gram positive bacteria were identified. There were even a few gram insensitive fellows that had some interesting characteristics such as being spiral shaped. Gram Staining Essay. Title: Identification of Two Unknown Bacteria’s Through the Use of Gram Staining Introduction: The Gram staining of bacteria is a widely used method to identify unknown bacteria.
The purpose of this method is to find out identities of unidentified bacteria. INTRODUCTION Gram staining is the most essential and universally used staining technique in bacteriology laboratory.
Page 1 of 2. treat and report the client based on the Gram stain results alone. It is a differential stain that allows you to classify bacteria as either gram.Gram staining essay